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KMID : 0624620130460080422
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BMB Reports
2013 Volume.46 No. 8 p.422 ~ p.427
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Activation of JNKs is essential for BMP9-induced osteogenic differentiation of mesenchymal stem cells
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Zhao Yan-Fang
Xu Jing
Wang Wen-Juan
Wang Jin
He Juan-Wen
Li Li
Dong Qian
Xiao Yan
Duan Xing-Lian
Yang Xue
Liang Yi-Wen
Song Tao
Tang Min
Zhao Dan
Luo Jin-Yong
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Abstract
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Although BMP9 is highly capable of promoting osteogenic differentiation of mesenchymal stem cell (MSCs), the molecular mechanism involved remains to be fully elucidated. Here, we explore the possible involvement and detail role of JNKs (c-Jun N-terminal kinases) in BMP9-induced osteogenic differentiation of MSCs. It was found that BMP9 stimulated the activation of JNKs in MSCs. BMP9-induced osteogenic differentiation of MSCs was dramatically inhibited by JNKs inhibitor SP600125. Moreover, BMP9-activated Smads signaling was decreased by SP600125 treatment in MSCs. The effects of inhibitor are reproduced with adenoviruses expressing siRNA targeted JNKs. Taken together, our results revealed that JNKs was activated in BMP9-induced osteogenic differentiation of MSCs. What is most noteworthy, however, is that inhibition of JNKs activity resulted in reduction of BMP9-induced osteogenic differentiation of MSCs, implying that activation of JNKs is essential for BMP9 osteoinductive activity.
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KEYWORD
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BMP9, Bone morphogenetic proteins, Mesenchymal stem cells, Mitogen activated protein kinases, Osteogenic differentiation
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